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Distinct stress and cell destruction pathways are engaged by TNF and ceramide during apoptosis of MCF-7 cells. — Alliance for NanoHealth
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You are here: Home Research Publications Distinct stress and cell destruction pathways are engaged by TNF and ceramide during apoptosis of MCF-7 cells.
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N J Donato and J Klostergaard (2004)

Distinct stress and cell destruction pathways are engaged by TNF and ceramide during apoptosis of MCF-7 cells.

Exp Cell Res. 294(2):523-33.

Department of Bioimmunotherapy, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

Ceramide has been proposed to be an important signaling intermediate in tumor necrosis factor (TNF)-induced apoptosis in human MCF-7 breast adenocarcinoma cells. We compared cell death and signal transduction pathways induced by TNF and ceramide in TNF-sensitive, parental MCF-7 cells to those in TNF-resistant, MCF-7 cells (3E9). TNF caused proteolysis of the caspase substrate, polyADP-ribose polymerase (PARP) in parental cells, but not in 3E9 cells. Both apoptosis and PARP cleavage were strongly prevented by co-incubation with caspase inhibitors. In contrast, ceramide-induced cell death was neither affected by TNF resistance nor was it associated with PARP cleavage, and death could not be prevented by co-incubation with caspase inhibitors in either cell line. TNF was able to activate JNK/SAPK approximately 30-fold and approximately 5-fold